Goodwin G. Jinesh

3 Questions 321 Answers 0 Followers

Questions related from Goodwin G. Jinesh

Anyone experienced CRISPR SpCas9-induced toxicity in mammalian cells?

I have transfected SpCas9 plasmid (Not viral transduction but lipofectamine transfection of lentiviral construct with SpCas9+PuroR) in human cancer cell lines, and the cells looked ok until...

14 April 2021 6,531 3 View

Does eosin stains collagen in IHCs in typical H&E staining?

Hi all, Does eosin stains collagen in IHCs in typical H&E staining? Please share your thoughts below as comments. Please add a reference if possible (much appreciated). Best, J.

23 May 2020 8,360 2 View

Are there any companies selling human ORF lenticlones with these tags EGFP/DsRed/mCherry/ECFP as fusion (not as GFP or RFP transduction reporters)?

If I wanted to buy alpha-Tubulin lentiviral clones it should be expressing EGFP-alpha tubulin as on transcript/protein not as EGFP separate and tubulin as separate proteins. Does anyone know a...

02 January 2013 2,109 4 View