Has anyone characterized human melanoma BRO cells? Can anyone please provide me with STR profile for this cell line or alternative means for its characterization? Cell repositories don't seem to have this information.
As BROs are not commonly used, I would be very grateful for any information you may give me.
Thank you!
Dear Tereza, thisarticles may be help you
We have analyzed the proteomes of two human melanoma cell lines (A375 and 526), and of the human melanocytes, (FOM 78), by two-dimensional electrophoresis (2D-PAGE) and liquid chromatography - tandem mass spectrometry (LC-MS/MS). Our comparative proteomic analysis revealed that six proteins were over-expressed in both melanoma cell lines as compared to melanocytes: galectin-1, inosine-5'-monophosphate dehydrogenase 2, serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform, protein DJ-1, cyclophilin A and cofilin-1. We show, for the first time, that only specific isoforms of these molecules are over-expressed in melanoma. Different protein profiles were also found between each individual melanoma cell line and the melanocytes. s-Methyl-5-thioadenosine phosphorylase, ubiquitin and ribosomal protein S27 a precursor, the basic form of protein DJ-1, annexin a1, proliferation associated protein 2g4, isoform alfa-enolase of alfa-enolase, protein disulfide-isomerase precursor, and elongation factor 2 were more strongly expressed in A375 cells compared to melanocytes. In 526 cells, 60s acidic ribosomal protein p1 and calreticulin precursor were more highly expressed than in melanocytes. These molecular differences may help in better understanding melanoma development and its different responsiveness to therapies. The identified proteins could be exploited as biomarkers or therapeutic targets for melanoma.
As you can see from the Cellosaurus entry we have not been able to find any STR data for the BRO cell line:
http://web.expasy.org/cellosaurus/CVCL_7036
And as you say BRO is not used commonly, so you may be luck if you ask labs that are using the derived cell line BLM if they have some STR profile for it. There is a recent paper of a group using BLM:
http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0134396
Good luck!
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