Can you help me with design of PCR amplification of long GC-rich regions?

Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...

24 August 2020 4,035 3 View

Can anyone recommend a corpus of research articles written by English native speakers?

I would like to use it as a reference corpus. I know there is a SERAC corpus, but it does not seem to be available for public.

01 April 2019 5,612 6 View

Illumina NextSeq multiplexing 384 samples per run? Barcodes?

Hello, I would like to ask you if you have any experience with multiplexing of 384 samples per run on Illumina NextSeq. We are using SNP assay designed by Roche = SeqCap EZ Choice library with...

05 December 2018 4,041 3 View

Can I use Tris/HCl instead to Bis-Tris in the gradient elfo with the same working pH and MES buffer?

I try a new method for chloroplasts analysis and its my first experiment with gradient electrophoresis for membrane proteins. In the protocol is Bis-Tris used, in lab we have Tris-HCl, the working...

04 December 2017 4,831 3 View

How to report results for generalised linear mixed model with binomial distribution?

Hello, I am currently working on the data analysis for my MSc. project comparing probability of occurrence of a species between two different habitats using presence - absence data. Due to the...

29 October 2017 8,273 5 View

Mapping ChiP-seq data on HiC data?

Hello, I am a bit new to the ChiP-seq data analysis and I need to map them onto HiC data, is there any tool to do that or visualize that somehow? Either online or using R-bioconductor? Any...

22 October 2017 8,782 1 View

Any database/online dictionary showing clearly morphemes and syllables (division)?

Hello everyone. I am urgently looking for a database or dictionary that shows already fragmented words - fragmented into morphemes and syllables as well (not pronounciation). I need it for a...

16 September 2017 8,583 1 View

Characterization for human melanoma BRO cell line?

30 April 2017 8,515 3 View

How to remove polysaccharides from protein samples?

Good afternoon, I try to extract cell wall proteins from green macroalgae, I have tried different protocols and one of them included step of EGTA extraction, however this fraction is jelly like,...

08 February 2017 1,271 2 View

What is the lifespan of an exosome in a cell culture medium?

26 August 2014 4,728 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View