Can you help me with design of PCR amplification of long GC-rich regions?

Hi everyone, I was wondering if anyone has a solution for the amplification of long GC-regions using PCR? I want to isolate ~5kb long sequence from mouse genome that is unfortunately rich in...

24 August 2020 4,035 3 View

Do you know about any questionnaires for children measuring their attitudes to science and scientists?

I am doing a qualitative study on children's attitudes to science and scientists before and after our science education intervention, we deliver to primary schools. I'd like to include a...

27 January 2020 8,001 3 View

Illumina NextSeq multiplexing 384 samples per run? Barcodes?

Hello, I would like to ask you if you have any experience with multiplexing of 384 samples per run on Illumina NextSeq. We are using SNP assay designed by Roche = SeqCap EZ Choice library with...

05 December 2018 4,041 3 View

Can I use Tris/HCl instead to Bis-Tris in the gradient elfo with the same working pH and MES buffer?

I try a new method for chloroplasts analysis and its my first experiment with gradient electrophoresis for membrane proteins. In the protocol is Bis-Tris used, in lab we have Tris-HCl, the working...

04 December 2017 4,831 3 View

How to report results for generalised linear mixed model with binomial distribution?

Hello, I am currently working on the data analysis for my MSc. project comparing probability of occurrence of a species between two different habitats using presence - absence data. Due to the...

29 October 2017 8,273 5 View

Mapping ChiP-seq data on HiC data?

Hello, I am a bit new to the ChiP-seq data analysis and I need to map them onto HiC data, is there any tool to do that or visualize that somehow? Either online or using R-bioconductor? Any...

22 October 2017 8,782 1 View

Any database/online dictionary showing clearly morphemes and syllables (division)?

Hello everyone. I am urgently looking for a database or dictionary that shows already fragmented words - fragmented into morphemes and syllables as well (not pronounciation). I need it for a...

16 September 2017 8,583 1 View

Characterization for human melanoma BRO cell line?

30 April 2017 8,515 3 View

How to remove polysaccharides from protein samples?

Good afternoon, I try to extract cell wall proteins from green macroalgae, I have tried different protocols and one of them included step of EGTA extraction, however this fraction is jelly like,...

08 February 2017 1,271 2 View

What is the lifespan of an exosome in a cell culture medium?

26 August 2014 4,728 3 View

How do I collect online measures in a same-different task?

The same-different task requires to subjects to indicate whether a pair of stimuli seen or heard are the same (say AA or BB) or different (say AB or BA). Researchers often collect offline measures...

20 February 2021 9,226 2 View

How can we assess applied digital skills and soft skills through online examinations during COVID-19 semester?

The final qualification assessment for major programs is a strict regimen that involves different stages - oral and written exams, final project viva, internal and external review. How are ICT...

16 February 2021 1,762 8 View

Participation in questionnaire for PhD Thesis, any English, Chinese or Spanish speakers?

Hello! I am looking for Spanish, English and Chinese native speakers to participate in my final survey for my PhD thesis. https://claudiomastrangelo4.wixsite.com/phdlinguist1/research This is...

01 February 2021 4,177 10 View

How can we use `Discourse Analysis` to analyze a literary text, more specifically a corpus of poems?

In fact, I need to know the analytical tools related to discourse analysis that might be used to tackle a literary discourse (precisely poems). I also need the names of the prominent linguists who...

26 January 2021 4,687 20 View

Corpus linguistics collocate indicative of diversity?

I have a question, as to whether collocations in corpus linguistics can be used to indicate diversity. I have a corpora of media news articles, and I manage to find out the frequency of my target...

19 January 2021 1,446 1 View

What course-book would you recommend for using to teach Manipulative Discourse and CDA to Bachelor students at a linguistic university?

I need recommendations for a course-book for teaching Manipulative Discourse and CDA to BA level students of Translation.

09 January 2021 645 6 View

Hi! What is the best way to define/specify content-related factors and style-related factors in terms of language variation / gender differences?

28 December 2020 4,409 3 View

No title

26 December 2020 9,337 3 View

Can language alter our perception of crime?

I am looking for any resources which may be useful in a study I am conducting on the impacts that language may have on our perception of crimes. I will be using headlines which convey a particular...

23 December 2020 8,713 6 View

Is there a future in the marriage of linguistics and sociology?

From the perspective of linguistics in general and sociolinguistics in particular what are the affordances of collaboration between linguists and sociologists?

06 December 2020 5,615 7 View