Dear all,
I want to set the experiment that the phosphorylation of my target protein in human cancer cell lines cannot be activated by the gene editing method.
How can I achieve this?
In simple, I am always think about how to change the status of phosphorylation of target protein using gene editing procedure, such as plasmid construction, CRISPR-CAS9, or other methods.
Thank you sincerely advance for your kind answers.
Dr. Zhou