13 Questions 3 Answers 0 Followers
Questions related from Dachen Zhou
Dear all, I want to active the phosphorylation of target protein in cell lines, but I do not want to use agent/drug, because the agent/drug may cause unexpected effects. How to do this? Thank you.
03 June 2017 8,010 1 View
Dear all, I want to build the Sorafenib Tosylate resistant hepatocarcinoma cell lines, HepG-2 and Hep-3B, does someone have experience about this? I have no experience about this, so I want to get...
06 April 2017 8,967 1 View
Dear all, For clinical data analysis, which analysis software is the best? R, SAS, SPSS, or STATE? I need to analysis the clinical data from more than 500 patients of HCC. Sometimes, I also need...
05 January 2017 5,006 5 View
Dear all, I want to knock down one protein by 50% in HCC cell lines. I want to know whether it is possible and how to achieve it? Thanks sincerely for your answer. Zhou.
01 December 2016 4,367 3 View
Dear all, Recently, one HCC patient of mine, his AFP level declined to the normal level three month later after liver resection. However, one month later, his AFP rebound and continue to increase....
08 October 2016 1,076 3 View
Dear All, Attachment is the image of one H&E staining of human liver tissue. I confused why the color is brown? The other slides in the same chamber are OK, except this one. Can someone tell...
17 September 2016 3,358 4 View
Now, I want to make paraffin wax with frozen tissue(Liver tissue). I check some information, it says that it is not suitable for frozen tissue to make paraffin wax, because tissue will be damaged...
05 August 2016 9,679 2 View
Recently, I met a patient with HCC recurrence, who received liver resection just 5 months ago, and followed with twice TACE treatment. The primary single tumor is small, just 2cm diameter. APF is...
02 July 2016 8,452 2 View
Currently, I want to check the function of protein A in fibroblast cell line and pancreatic cancer cell lines. Is it possible in theory? For example, I want to check the expression of protein A in...
24 March 2016 2,278 3 View
In my project, we find one agent which can down regulate phosphorylation of 4EBP1, so we think this agent maybe can inhibit mTOR. We tried to active mTOR to check this function of agent. We used...
26 October 2014 786 6 View
I want to find whether the test drug can activate mTORC1 but not activate mTORC2. I have checked the expression of p70s6 and 4ebp1, now, how can I check the activation of mTORC2?
14 October 2014 8,745 12 View
Dear all, I want to set the experiment that the phosphorylation of my target protein in human cancer cell lines cannot be activated by the gene editing method. How can I achieve this? In simple, I...
01 January 1970 2,179 1 View
Dear all, I want to ask the company to make the tissue array with our own liver tissue (HCC) from our tissue bank. Can someone tell me how many sample should be on one slide? I mean how to design...
01 January 1970 4,743 1 View
