Can positive cell screening after stable transfection be combined with two or more antibiotics to screen cells? Will all the cells die?
Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...
23 June 2024 4,902 3 View
To examine the impact of community engagement in heritage preservation and interpretation
01 June 2024 3,478 11 View
Hello every, I am looking for a commercial enzyme that can degrade sulfate dextran. What is known at present is that dextran can be a substrate for dextranase. so I want to know if sulfate...
23 May 2024 1,471 0 View
In order to understand whether the two newspapers marginalised one group and diverted attention from other group following a case study. Firstly using first and second levels of Fairclough’s...
23 May 2024 3,208 5 View
I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?
21 May 2024 9,684 1 View
Do embryonic stem cells or iPSCs cultured in vitro on normal culture plates migrate spontaneously without any stimulation?
18 May 2024 6,861 2 View
The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...
15 May 2024 1,458 3 View
As the known, there were the most richness species diversity of the cave animals, especially for the cave fishes, because the underground river sytems. But there were the diffculties exist also,...
14 May 2024 9,131 0 View
Hello, everyone, recently I have been transferring two-dimensional materials, using PC transfer, but there is a problem with the configuration of PC solution recently, and I cannot pick up the...
11 May 2024 6,564 0 View
We use DpnI to digest PCR product(already purified),then after digesting,do I need purified again?
09 May 2024 2,499 3 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
EDX analisys
31 July 2024 5,947 2 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I am working on algal extract to which gas chromatography (Not GC-MS) spectrum I want to discover. My question is can we identify specific compounds using retention time if I compared the RT with...
29 July 2024 8,034 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View