chang liver cells and liver?

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Can anyone tell me the relationship of gene expression in base pairs and amplicon size?

I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.

09 October 2017 6,393 0 View

Can anyone suggest me a program which can be used to check whether a particular gene sequence may be working or not?

As par of my research work, I have to deal with the expression patterns of certain genes. I came across different sequences for same genes in different publications. So can anyone please suggest...

10 November 2016 5,411 7 View

Did anyone know whether Heart type fatty acid binding proteins and Liver fatty acid binding proteins are present in HL-60 cells?

I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.

08 September 2016 9,340 1 View

Can anyone suggest me an useful software that can be used to check whether a given DNA sequence is working or not?

As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...

08 September 2016 5,252 4 View

Can Propidium Iodide uptake assay be done in rat heart/ liver tissues?

As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.

07 August 2016 6,272 3 View

Can anyone suggest me some good kits for performing TUNEL assay in vivo?

As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?

07 August 2016 3,108 2 View

Can anyone provide me the calculation for SOD using the Method of Kakkar et al., (1984)?

I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...

06 July 2016 3,430 1 View

Can anyone please suggest to me a method for isolating cell membranes?

I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...

02 March 2016 6,640 1 View

Is there any simple method which serves as a marker assay for inflammation instead of COX-2 and LOX-5?

Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.

11 December 2015 7,032 4 View

Is it suitable to carry out centrifugation in normal centrifuges (instead of cooling centrifuge) while doing enzymatic assays?

Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.

10 November 2015 8,325 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View