Can anyone tell me the relationship of gene expression in base pairs and amplicon size?

I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.

09 October 2017 6,242 0 View

Can anyone suggest me a program which can be used to check whether a particular gene sequence may be working or not?

10 November 2016 5,294 7 View

Can anyone suggest me an useful software that can be used to check whether a given DNA sequence is working or not?

As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...

08 September 2016 5,137 4 View

Can Propidium Iodide uptake assay be done in rat heart/ liver tissues?

As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.

07 August 2016 6,155 3 View

Can anyone suggest me some good kits for performing TUNEL assay in vivo?

As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?

07 August 2016 2,971 2 View

Can anyone provide me the calculation for SOD using the Method of Kakkar et al., (1984)?

I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...

06 July 2016 3,290 1 View

Can anyone please suggest to me a method for isolating cell membranes?

I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...

02 March 2016 6,495 1 View

Is there any simple method which serves as a marker assay for inflammation instead of COX-2 and LOX-5?

Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.

11 December 2015 6,901 4 View

Is it suitable to carry out centrifugation in normal centrifuges (instead of cooling centrifuge) while doing enzymatic assays?

Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.

10 November 2015 8,195 4 View

Can anyone suggest me methods for identifying COX-2 and LOX-5 without using kits?

Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...

10 November 2015 9,824 6 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View