I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.
As par of my research work, I have to deal with the expression patterns of certain genes. I came across different sequences for same genes in different publications. So can anyone please suggest...
10 November 2016 5,411 7 View
I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.
08 September 2016 9,340 1 View
As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...
08 September 2016 5,252 4 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
07 August 2016 6,272 3 View
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
07 August 2016 3,108 2 View
I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...
06 July 2016 3,430 1 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
02 March 2016 6,640 1 View
Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.
11 December 2015 7,032 4 View
Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.
10 November 2015 8,325 4 View
Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...
10 November 2015 9,961 6 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...
30 July 2024 841 2 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?
27 July 2024 6,920 1 View
Gene sequencing related trouble shooting
25 July 2024 4,149 2 View