19 Questions 11 Answers 0 Followers
Questions related from Abhilash S
I wish to know is it possible to correlate the expression of a gene in base pairs with its amplicon size.
10 October 2017 6,352 0 View
As par of my research work, I have to deal with the expression patterns of certain genes. I came across different sequences for same genes in different publications. So can anyone please suggest...
11 November 2016 5,382 7 View
I am working on some fatty acids. I wish to know whether FABPs of Heart and Liver types are present in HL-60 cells.
09 September 2016 9,306 1 View
As part of my research work, I have to check the expression of some genes. However when I go through references, I got different sequences for the same gene. I wish to know which of these...
09 September 2016 5,222 4 View
As part of my research work, I have to perform apoptosis assay with animal tissues. I wish to know, whether Propidium Iodide uptake assay be done in rat heart/ liver tissues.
08 August 2016 6,243 3 View
As part of my research work, I have to perform TUNEL assay in in vivo. Can anyone suggest me some good kits which are economical, based on their experience?
08 August 2016 3,055 2 View
I am working with the effect of certain chemicals in antioxidant system using Cell line models. As part of my work, I have to perform SOD assay. I choose the method of Kakkar et al., (1984), since...
07 July 2016 3,396 1 View
I am working with cell lines. As part of my work, I wish to isolate the membranes of cells. I wish to know if there is any simple method for cell membrane isolation which can be done without using...
03 March 2016 6,598 1 View
Hello, I am working on cell culture. I wish to know if there is any simple method which serve as marker of inflammation instead of Cyclooxygenases and lipooxygenases.
12 December 2015 6,990 4 View
Hello, I am working on cell culture and wish to perform inflammatory assays, especially, COX and LOX. I have found several kits of different companies. Due to financial limitations, I think its...
11 November 2015 9,923 6 View
Hello, I wish to know Whether it is good to carry out centrifugation in normal centrifuges instead of cooling centrifuges while doing enzymatic assays especially in cell culture.
11 November 2015 8,284 4 View
Hello, I bought Amphotericin B from a company. Their instruction is to dissolve it in DMSO at a concentration of 3 mg/mL. Is it suitable to add 10 microliter of this solution (ie 6 micro gram of...
10 October 2015 1,630 5 View
I wish to get expert opinion about the chang cells as a model for liver.
06 June 2015 2,472 2 View
I wish to know if there is any chemical change due to repeated sterilization of buffer solutions.
03 March 2015 9,980 2 View
I wish to publish data on work related to toxicity. Can anyone please list me some useful peer reviewed journals in Biology/ Life sciences that do not have publication charge?
11 November 2014 9,640 6 View
Can anyone please tell me the best anti fungal agent that can be used for the removal of mold contamination from cell culture media and also the required concentration of the anti fungal agent per...
11 November 2014 7,006 8 View
Can anyone please tell me the effective concentration of trypsin used for trypsinization of H9c2 cells? I had used 1x trypsin from Sigma and got good results. But now I got 10x trypsin and it did...
10 October 2014 5,432 3 View
I have a doubt regarding the expression of Nrf2 by cardiomyocytes and hence I wish to make it clear.
08 August 2014 6,836 4 View
I have been working with some expression studies and has found some publications with and some others without ladder. Can anyone help me?
07 July 2014 6,889 3 View
