How can I take subsamples of water from a larger sample in a way that allows me to measure the iron content of the subsample?

I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...

04 February 2021 4,778 1 View

Can anyone recommend a good ICC antibody specific for human TMEM119?

Mouse antibodies on the market appear to be better than the human antibodies, which seem to label poorly. Any experience with antiTMEM119 antibodies for labeling human microglia would be helpful....

22 July 2020 7,279 1 View

Is there a microglia specific marker to differentiate from macrophages (that is not TMEM119)?

Hello - I'm working with stem cell derived microglia. When adding to 3D cultures I'm not getting a signal for TMEM119, which based on literature could be due to the immaturity of the cells (where...

19 February 2020 1,543 3 View

Synthetic Materials with Acoustic Impedances of 1.53 and 1.69?

Hello, My name is Colleen O'Brien, a senior biomedical engineering student.For my senior project I am required to build a model of human abdomen to validate a device we are developing. I still am...

04 December 2019 3,275 3 View

Mosaic Image J interaction analysis Hernquist strength?

I'm trying to use Mosaic Image J for interaction Analysis of pre and post synaptic puncta. When running the program it gives measurements for "Strength, Residual, and Scale." Can someone explain...

06 January 2019 4,194 1 View

Clumped co-culture cells cause?

Has anyone dealt with technical issues with both primary rat neuron/glia co cultures and human iPSC neuron astrocyte co cultures (both on PDL), where the cells seem to ball up near the cell body...

18 November 2018 8,787 4 View

Is there an information overload theory?

I am writing an introduction about information overload. As of now I can't find any theory about information overload. Is there one? Better if it is related to library and information science....

18 February 2018 1,267 13 View

What statistical test should I do for sholl analysis (neuronal dendrite complexity) with 2 independent variables?

I have 2 independent variables (genotype and treatment) and I want to analyze their correlation though sholl analysis. Which statistical test should I do?

02 November 2016 6,409 3 View

Can anyone recommend a well recognized online epidemiology course?

Our institution does not have such a course and I would like to become credentialed for epidemiology.

12 November 2013 962 10 View

Your Professional Legacy

How do you want your students to remember you?

01 January 1970 3,600 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to choose ANN number of hidden layers and nodes?

Hello, Could you please share any interesting research explaining how to choose the number of hidden layers and nodes per layer in case of regression problems using ANN? Thank you, any help would...

01 March 2021 6,200 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View