Looking for a good microglia-specific promoter, other than TMEM119, for the purpose of plasmid design, to be packaged in AAVs. In vitro and in vivo applications.
There is a USEPA standard on this I believe, but I am having trouble finding it. Our lab washes dishes and we place dried dishes in the HCl acid bath to remove contamination but focus especially...
09 August 2023 2,031 4 View
Tg vs stiffness and elastic modulus
20 July 2023 9,382 1 View
I am working on a research about developing an information literacy program module for high school students.
25 September 2021 6,962 6 View
I am using a Beckman ultracentrifuge to settle out colloids (less than 1um in diameter, with filtration occurring beforehand) from soil column leachate and trying to figure out the lower limit of...
12 May 2021 1,260 0 View
I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...
04 February 2021 5,152 1 View
I am planning to analyze manure samples for Total Kjeldahl NItrogen, Total Kjeldahl Phosphorus, and dissolved reactive phosphorus using and AQ2 analyzer. I would like to also look at...
27 August 2020 7,056 4 View
Mouse antibodies on the market appear to be better than the human antibodies, which seem to label poorly. Any experience with antiTMEM119 antibodies for labeling human microglia would be helpful....
22 July 2020 7,380 1 View
Hello - I'm working with stem cell derived microglia. When adding to 3D cultures I'm not getting a signal for TMEM119, which based on literature could be due to the immaturity of the cells (where...
19 February 2020 1,657 3 View
Hello, My name is Colleen O'Brien, a senior biomedical engineering student.For my senior project I am required to build a model of human abdomen to validate a device we are developing. I still am...
04 December 2019 3,467 3 View
I'm working on a co-localization algorithm in CellProfiler based on Neiland 2014. After filtering and making the image binary - I get two different images for Homer1 and SYT1 when identifying the...
02 September 2019 1,607 0 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...
31 July 2024 225 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View