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Questions related from Colleen S Limegrover
Hello - I'm working with stem cell derived microglia. When adding to 3D cultures I'm not getting a signal for TMEM119, which based on literature could be due to the immaturity of the cells (where...
20 February 2020 1,608 3 View
I'm working on a co-localization algorithm in CellProfiler based on Neiland 2014. After filtering and making the image binary - I get two different images for Homer1 and SYT1 when identifying the...
03 September 2019 1,578 0 View
I'm trying to use Mosaic Image J for interaction Analysis of pre and post synaptic puncta. When running the program it gives measurements for "Strength, Residual, and Scale." Can someone explain...
07 January 2019 4,258 1 View
Has anyone dealt with technical issues with both primary rat neuron/glia co cultures and human iPSC neuron astrocyte co cultures (both on PDL), where the cells seem to ball up near the cell body...
19 November 2018 8,875 4 View
Does anyone know of companies who sell or do custom PDL coating for 384 well plates? Other than the big obvious choices (Greiner, Corning, Phoenix)? Looking for options for primary neuronal cells.
26 June 2017 944 1 View
I'm wondering if anyone has insight into the effect of minimal changes in CO2 incubator levels on primary neuronal cell cultures? Cells are plated at a density of 4.66104 cells per cm2 in 384-well...
23 March 2017 5,176 1 View
Does anyone know the brain concentration or % of antibody that was able to pass the BBB in patients treated with Abeta antibodies bapineuzumab or solanezumab? Thank you.
21 December 2016 2,159 3 View
