Mouse antibodies on the market appear to be better than the human antibodies, which seem to label poorly. Any experience with antiTMEM119 antibodies for labeling human microglia would be helpful. Thanks!
When in doubt or trying something new, I always look at antibodypedia
Link below...
https://www.antibodypedia.com/
I learned how to take subsamples with a magnetic stir bar, but I noticed iron particles stick to the bar, and I am having trouble finding a standard method to solve that problem. How can I take a...
04 February 2021 4,778 1 View
Hello - I'm working with stem cell derived microglia. When adding to 3D cultures I'm not getting a signal for TMEM119, which based on literature could be due to the immaturity of the cells (where...
19 February 2020 1,543 3 View
Hello, My name is Colleen O'Brien, a senior biomedical engineering student.For my senior project I am required to build a model of human abdomen to validate a device we are developing. I still am...
04 December 2019 3,275 3 View
I'm trying to use Mosaic Image J for interaction Analysis of pre and post synaptic puncta. When running the program it gives measurements for "Strength, Residual, and Scale." Can someone explain...
06 January 2019 4,194 1 View
Has anyone dealt with technical issues with both primary rat neuron/glia co cultures and human iPSC neuron astrocyte co cultures (both on PDL), where the cells seem to ball up near the cell body...
18 November 2018 8,787 4 View
I am writing an introduction about information overload. As of now I can't find any theory about information overload. Is there one? Better if it is related to library and information science....
18 February 2018 1,267 13 View
I have 2 independent variables (genotype and treatment) and I want to analyze their correlation though sholl analysis. Which statistical test should I do?
02 November 2016 6,409 3 View
Our institution does not have such a course and I would like to become credentialed for epidemiology.
12 November 2013 962 10 View
How do you want your students to remember you?
01 January 1970 3,600 4 View
How do you foster discourse (around the subject you teach) which enables the student to continue to acquire the necessary knowledge of their subject area and their role in the world?
01 January 1970 9,846 6 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
02 March 2021 3,024 2 View
Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...
01 March 2021 9,536 3 View
As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...
01 March 2021 3,199 3 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View