How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

How to identifying & charecterization of lactobacillus & Bifidobacterium? ??

25 July 2017 9,836 2 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

Can any one suggest suitable reference or procedure to find presence of elements or compounds from XRD peaks in Xpert high score plus software ?

Sample is ZrB2 (Zirconium Diboride )powder.

01 May 2017 8,424 1 View

How to make the gold nanocubes (CTAC stabilized) surface negatively charged ?

I have gold nano cubes made using CTAC .For some of my studies i need negatively charged gold nano cubes .can anyone suggest me how i can make the surface charge negative.which method i can use it...

10 April 2017 8,773 1 View

Does anyone have protocol of amylase assay for bacteria..?

05 April 2017 2,922 23 View

Can anyone suggest a tool or software which can efficiently convert GTF file formats to GFF?

I want to use software which takes GFF file formats as input, but I've got GTF files. http://www.sequenceontology.org fails. So if anyone can provide me with a script or suggest tools, that would...

08 April 2014 8,532 4 View

Which software can produce a visual splice graph of splice variants?

I have collected all known splice variants of few genes and need to produce a visual splice graph that tells which exons are missing or trimmed or extended in which variants. Which software/tool...

25 February 2014 756 4 View

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02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View