What should the village government do in dealing with the spread of the covid-19 virus?

I am currently researching village government policies regarding the spread of the covid-19 virus, I want to gather opinions from various villages or areas affected by the Covid-19 virus, what...

07 September 2020 303 5 View

What are the importance of genetic tests to detect cancer?

Cancer is not a single disease with a single type of treatment. There are more than 200 different types of cancer, each with its own name and treatment. Medical tests can look for many inherited...

21 March 2019 209 5 View

How can PLGA be solidified while making composite with hydroxyapatite?

Hello people, I have been trying to develop composites from PLGA (Molar ratio- 50:50) with hydroxyapatite by solvent casting technique. But the slurry formed by mixing the two components does not...

27 December 2018 8,313 2 View

Why there is a decrease in the intensity and merging of xrd peaks of Hydroxyapatite after soaking it in SBF solution for 28 days?

I have prepared Hydroxyapatite pellets and immersed it in SBF solution for 28 days. The xrd analysis of post SBF Hydroxyapatite shows decrease in the intensity of peak and merging of some peaks...

20 November 2018 4,271 10 View

What are the challenges and outlook of biomass utilization in Metallurgical Industries?

One of the great challenges is to find a sustainable way to obtain biobased products from renewable resources. A significant emphasis has been on the developmental biorefinery concept and...

28 July 2018 2,346 3 View

How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

What are the new improvements that make LED a powerful tool for lighting systems optimized for photosynthesis?

24 November 2017 9,935 5 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

Can any one suggest suitable reference or procedure to find presence of elements or compounds from XRD peaks in Xpert high score plus software ?

Sample is ZrB2 (Zirconium Diboride )powder.

01 May 2017 8,424 1 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View