You should the raman spectroscopy measurement at least twice for both stress and unstress (it gives you better results if you do it for several states where you have different levels of stress). The Raman peak shifts for samples with stress comparing to the case without stress. The difference between the peaks is proportional to the eigenvalues of the secular equation, which relates the Raman peaks to the stress state. Noted that there are two transverse modes and one longitudinal mode, which you must consider all.
Here this manuscript provides the formulas that you need for your calculation.
You can calculate the stress in single layer graphene by knowing the shift of the 2D peak, with respect to its position for a relaxed graphene sheet. The following publication may help you