How to promote the adhesion of biological matrices onto slides for histology?

Hi all, I am using animal-derived biological matrices as support for cell culture. At the end of the experiment I process the scaffolds+cells for histological examination. I include the samples...

05 June 2015 1,091 4 View

Does anyone know a good antibody anti-human nuclei?

I want to recognize human cells transplanted in mice and I'm working with OCT-frozen tissues.

08 September 2014 1,032 4 View

Measurement of Nitric Oxide in Cell supernates

I want to measure the total nitric oxide produced by my cells and released as nitrites + nitrates in the culture medium. I'm trying to use a Nitrate/Nitrite Assay colorimetric kit, but I'm having...

03 April 2013 2,595 11 View

How can I culture and expand mouse cardiac Sca1+ stem cells?

I'm isolating Sca-1+ stem cells from mice hearts and I would like to expand the cells in culture before using them for my experiments. Can someone kindly suggest me how to cultivate these cells?...

01 February 2013 5,703 3 View

Stem cells from diabetic subjects

If I isolate stem cells from diabetic subjects (mice or humans) do these cells retain a memory of the hyperglycaemic state also after several passages in culture in basal glucose medium?

31 December 2012 6,476 15 View

In-vitro model of hyperglycaemia: what is the appropriate D-glucose concentration?

In your experience, what is the best concentration of D-glucose to use in human cell cultures to mimic the diabetic condition? I'm going to use 30mM D-Glucose, but I know that someone thinks 25mM...

31 December 2012 4,838 15 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View