7 Questions 26 Answers 0 Followers
Questions related from Jitendra Singh
Please provide name of the the dealers / supplers for hERG (human ether-à-go-go-related gene) transfected either CHO or HEK-293 cells. I got some quotaions seems to be very expensive. we need for...
05 May 2018 5,270 3 View
I am using JC 10 stained for mitochondrial membrane potential. if anybody is using can you please suggest me the protocol for staining with JC-10 dye. thanks in advance
10 October 2016 2,977 1 View
I am trying to optimize the transfection by using lipofectamine 2000 for 2000 ng of plasmid DNA/ well with different amounts of lipofectamine 2000 (5ul, 10ul) in the 6-well plate. There is a...
04 April 2016 6,652 6 View
plese provide the protocol how we can go through transfection of the cell using the GFP tag etc, positive control of the expression system to check the expression of the channel?
02 February 2016 8,606 4 View
I am recording the sodium currents in whole cell patch clamp methods in DRG neurons (20 micrometer) from the Axopatch-200B and getting capacitance 1.24 pF in the dial. Should the capacitance be...
03 March 2014 10,087 9 View
I want to study the effect of long term treatment (14 days and 28 days) of a compound in olfactory bulbectomized (OBx) rats on locomotor activity (open field), aggressive behavior and sprouting...
04 April 2011 9,843 2 View
I am affraod to ask if any one suggest me How we can normalized the sodium current recorded from the whole cell configurations and calculation of tau value. Iam normalizing with positive value of...
03 March 2010 3,424 0 View
