How to promote the adhesion of biological matrices onto slides for histology?

Hi all, I am using animal-derived biological matrices as support for cell culture. At the end of the experiment I process the scaffolds+cells for histological examination. I include the samples...

05 June 2015 1,091 4 View

Does anyone know a good antibody anti-human nuclei?

I want to recognize human cells transplanted in mice and I'm working with OCT-frozen tissues.

08 September 2014 1,032 4 View

Measurement of Nitric Oxide in Cell supernates

I want to measure the total nitric oxide produced by my cells and released as nitrites + nitrates in the culture medium. I'm trying to use a Nitrate/Nitrite Assay colorimetric kit, but I'm having...

03 April 2013 2,595 11 View

Cardiomyocytes in vitro culture

I've just started working with adult rat cardiomyocytes. Can someone kindly suggest to me which is the best density for seeding these cells? I was thinking to plate 2000 cells in each well of the...

01 February 2013 7,663 2 View

Stem cells from diabetic subjects

If I isolate stem cells from diabetic subjects (mice or humans) do these cells retain a memory of the hyperglycaemic state also after several passages in culture in basal glucose medium?

31 December 2012 6,476 15 View

In-vitro model of hyperglycaemia: what is the appropriate D-glucose concentration?

In your experience, what is the best concentration of D-glucose to use in human cell cultures to mimic the diabetic condition? I'm going to use 30mM D-Glucose, but I know that someone thinks 25mM...

31 December 2012 4,838 15 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View