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Hi all, I am using animal-derived biological matrices as support for cell culture. At the end of the experiment I process the scaffolds+cells for histological examination. I include the samples...
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I want to recognize human cells transplanted in mice and I'm working with OCT-frozen tissues.
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I want to measure the total nitric oxide produced by my cells and released as nitrites + nitrates in the culture medium. I'm trying to use a Nitrate/Nitrite Assay colorimetric kit, but I'm having...
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I've just started working with adult rat cardiomyocytes. Can someone kindly suggest to me which is the best density for seeding these cells? I was thinking to plate 2000 cells in each well of the...
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I'm isolating Sca-1+ stem cells from mice hearts and I would like to expand the cells in culture before using them for my experiments. Can someone kindly suggest me how to cultivate these cells?...
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In your experience, what is the best concentration of D-glucose to use in human cell cultures to mimic the diabetic condition? I'm going to use 30mM D-Glucose, but I know that someone thinks 25mM...
01 January 2013 4,873 15 View
If I isolate stem cells from diabetic subjects (mice or humans) do these cells retain a memory of the hyperglycaemic state also after several passages in culture in basal glucose medium?
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