How to promote the adhesion of biological matrices onto slides for histology?

Hi all, I am using animal-derived biological matrices as support for cell culture. At the end of the experiment I process the scaffolds+cells for histological examination. I include the samples...

05 June 2015 1,091 4 View

Measurement of Nitric Oxide in Cell supernates

I want to measure the total nitric oxide produced by my cells and released as nitrites + nitrates in the culture medium. I'm trying to use a Nitrate/Nitrite Assay colorimetric kit, but I'm having...

03 April 2013 2,595 11 View

How can I culture and expand mouse cardiac Sca1+ stem cells?

I'm isolating Sca-1+ stem cells from mice hearts and I would like to expand the cells in culture before using them for my experiments. Can someone kindly suggest me how to cultivate these cells?...

01 February 2013 5,703 3 View

Cardiomyocytes in vitro culture

I've just started working with adult rat cardiomyocytes. Can someone kindly suggest to me which is the best density for seeding these cells? I was thinking to plate 2000 cells in each well of the...

01 February 2013 7,663 2 View

Stem cells from diabetic subjects

If I isolate stem cells from diabetic subjects (mice or humans) do these cells retain a memory of the hyperglycaemic state also after several passages in culture in basal glucose medium?

31 December 2012 6,476 15 View

In-vitro model of hyperglycaemia: what is the appropriate D-glucose concentration?

In your experience, what is the best concentration of D-glucose to use in human cell cultures to mimic the diabetic condition? I'm going to use 30mM D-Glucose, but I know that someone thinks 25mM...

31 December 2012 4,838 15 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there any research regarding how training and development is going to change in the era of Fourth Industrial Revolution?

As the organizations getting prepared for the fourth industrial revolution, to enhance the skills of their workforce what type of changes are necessary and going to occur in the training and...

01 March 2021 3,199 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View