Separation of protein via Akta system gave broad peak during elution. I wonder is it normal to obtain broad peak ?

Greetings.. I am using Akta Pure system for my protein purification. I have optimized buffer system for protein elution. However, this time I got broad peak while eluting sample. For...

24 December 2020 393 7 View

Can anybody suggest safest irreversible inhibitor for cholinesterase (EC:3.1.1.7)?

I am working on the assay of brain acetylcholine. I need an irreversible inhibitor of cholinesterase that is relatively safer (not volatile, preferably solid) to use. This is to prevent the...

08 December 2020 3,797 3 View

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. What it could be?

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. I have attached figure for the convenience. Suggestion and answers in this regard will be highly...

28 October 2020 2,869 1 View

Desired membrane protein band isn't aligned with marker in SDS-PAGE gel. What could be the possible reason?

I am having problems regarding to protein separation via SDS-PAGE. As a newbie in this field, I have tried to optimize the conditions for protein induction. My protein size is in 20-25KDa range...

03 September 2020 8,243 11 View

How to get an XRD peak for 2nd coat (MgF2), which is coated on Cadmium Telluride glass substrate?

We coated Magnisum Fluoride (1.5 Molar) solution and Spin coated on Cadmium Telluride (CdTe) glass plate. Anealed (heated in oven) at 70*Celcius for 2 hours and then sent for XRD. But we got only...

19 August 2020 1,678 3 View

How do I make Fuzzy Rules in Python Language?

Hi all, I am working on my Final Year Project whose title is Expert System for Assessing Programming Course Performance of E-Learning Students. We have to use Fuzzy Logic Algorithm of Artificial...

10 May 2020 1,819 13 View

Anyone using REDExtract-N-Amp kit for small insects?

Is anybody using REDExtract-N-Amp kit for DNA extraction from small insects? I am facing problems in PCR amplification with this kit. Any solution?

02 April 2020 9,667 2 View

PLA(Poilylactic acid) sheet suppliers in pakistan ?

Does anyone know a supplier company provide PLA (Polylactic-acid) plastic sheets in pakistan? There's several companies products Lactic acid-(food suppliers in liquid form, This is not what I...

16 March 2020 724 3 View

Fail Safe N, Orwin method?

I need to calculate Fail-Safe N of my meta-analysed studies. I am having difficulty in understanding Orwin's method. Can anyone please elaborate with the help of an example?

03 March 2020 7,403 3 View

Usefulness of CMA lite version?

Can I do moderator analysis on the lite version of Comprehensive meta-analysis software (CMA)? Or is it necessary to get the Pro version of it to perform moderator analysis? Quick responses would...

02 March 2020 328 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View