can any budy explain how i calculate the DNA concentration for the 50ul reaction?
Nadeem Khawar
Given information:
- Concentration of DNA is 85 ng/μl.
Step 1: Determine the recommended DNA concentration range for Phusion Flash Master Mix.
According to the Phusion Flash Master Mix product information, the recommended DNA template concentration range is 1-100 ng per 50 μl reaction.
Step 2: Calculate the amount of DNA required for a 50 μl reaction.
Amount of DNA required = Recommended DNA concentration range × Reaction volume
Amount of DNA required = 1 ng/μl × 50 μl = 50 ng
Step 3: Calculate the volume of DNA required for a 50 μl reaction.
Volume of DNA required = Amount of DNA required / DNA concentration
Volume of DNA required = 50 ng / 85 ng/μl = 0.588 μl
Therefore, for a 50 μl reaction using Phusion Flash Master Mix, you would need to add 0.588 μl of the DNA sample with a concentration of 85 ng/μl.
Good luck; partial credit AI
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