Can you suggest some sites where I could acquire proper understanding of electrochemistry and electrochemical detecton of DNA methylaton at a same time?
I was extracting miRNA from lung cancer cell(A549) derived exosome by Total Exosome RNA & Protein Isolation Kit from Invitrogen™. I quantified the small RNA concentration with NanoDrop just...
07 August 2019 7,317 5 View
I know that DEPC treatment can make water RNase free. If I use DEPC-treated water for making PBS, I think, at the time of checking the pH the solution may be contaminated with RNase. Is it...
06 July 2019 1,504 9 View
Suppose within body fluid (e.g., blood) there are exosomes with specific marker protein to be detected. I want to know what type of molecule might have the possibility to interacts with the...
08 September 2018 6,015 0 View
Run > Run Autogrid > launch > Error reports in *python shell*
10 November 2014 5,859 3 View
During preparation of .pdbqt file I had a warning like that : Total kollman charge added = -166.952. What's that? explain please.
10 November 2014 9,590 4 View
Enveloped viruses are formed by budding through cellular membranes, usually the plasma membrane but sometimes an internal membrane such as the ER, golgi, or nucleus. cell membrane composed of...
10 November 2014 4,618 3 View
I want an alignment of approx. 100 sequence considering 100% similar sequence only once.
10 November 2014 4,164 7 View
I'm new to autodock. I need proper concept of autodock as well as some tutorial with example.
10 November 2014 7,182 4 View
In Malate-aspartate shuttle oxaloacetate first convert to malate and then pass inner mitochondrial membrane. in matrix of mitochondria oxaloacetate regenerated from malate.
07 August 2014 6,658 7 View
I was studying a paper titled ''Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.'' There I found the term cell line isolate...
01 January 1970 8,704 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...
01 August 2024 2,079 0 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
In my molecule there is Chloro group at 2-position of phenyl ring, but in 2D image it appears as methyl showing no interaction.
28 July 2024 734 0 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,983 2 View
Hi, Now I am doing a single-base editing by using HiFi Cas 9, guideRNA, single stranded DNA, HDR Enhancer V2 and my tranfection mehtod is lipofection (RNAiMax). Unfortunately, after gene editing,...
26 July 2024 2,136 0 View
Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...
25 July 2024 4,927 3 View