How much microRNA concentration is being reported just after extraction from exosome?

07 August 2019 7,275 5 View

How to make RNase free PBS buffer?

I know that DEPC treatment can make water RNase free. If I use DEPC-treated water for making PBS, I think, at the time of checking the pH the solution may be contaminated with RNase. Is it...

06 July 2019 1,471 9 View

Can you suggest some tutorials of electrochemical detecton of DNA methylation?

Can you suggest some sites where I could acquire proper understanding of electrochemistry and electrochemical detecton of DNA methylaton at a same time?

09 October 2015 6,655 0 View

Autogrid launching generating error?

Run > Run Autogrid > launch > Error reports in *python shell*

10 November 2014 5,825 3 View

Can someone help with a "Target.pdbqt" preparation problem?

10 November 2014 9,564 4 View

What does viral envelope contains: lipid bilayer or phospholipid bilayer?

Enveloped viruses are formed by budding through cellular membranes, usually the plasma membrane but sometimes an internal membrane such as the ER, golgi, or nucleus. cell membrane composed of...

10 November 2014 4,583 3 View

Where can I find an automatic alignment software?

I want an alignment of approx. 100 sequence considering 100% similar sequence only once.

10 November 2014 4,129 7 View

Autodock tutorial for beginners?

I'm new to autodock. I need proper concept of autodock as well as some tutorial with example.

10 November 2014 7,149 4 View

Why can't oxaloacetate pass through inner mitochondrial membrane?

In Malate-aspartate shuttle oxaloacetate first convert to malate and then pass inner mitochondrial membrane. in matrix of mitochondria oxaloacetate regenerated from malate.

07 August 2014 6,624 7 View

What is ''stringent cell line''?

I was studying a paper titled ''Proteomic profiling of NCI-60 extracellular vesicles uncovers common protein cargo and cancer type-specific biomarkers.'' There I found the term cell line isolate...

01 January 1970 8,671 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Why does two different bioinformatics tools for the same proteins show different subcellular localizations?

During a computational analysis, I found that for a protein of a plant, two different subcellular localizations are seen using CELLO and WoLF PSORT. For example, in CELLO, it identified the...

01 March 2021 6,729 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

What is the difference between genotype and haplotype data in the ped format?

Hi everybody In the ped format for genotype, alleles of any SNP are represented by two columns (one for each allele, separated by a space). Is a column sufficient for the haplotype to...

27 February 2021 1,965 1 View