PFA6a - mRFP - kanMX6 - plasmid whether integrative or replicative plasmid??

Brundha Annadurai @Brundha-Annadurai

12 March 2018 1 737 Report

pFA6a - mRFP - kanMX6 - plasmid whether integrative or replicative plasmid??
How to confirm, whether this RFP (Red fluorescent protein) plasmid integrative or replicative?

Ziguo Zhang

integrative

Badges
Science topic

More Brundha Annadurai's questions See All

How to fix this error in AutoDock vina"ValueError: Could not find atomic number for Ha Ha"?

Dear all, I am using AutoDock Vina to obtain the binding free energy of a ligand and a crystal surface. Using ADFR suites to convert input file from pdb to pdbq, I use prepare_receptor -r...

04 December 2023 9,970 0 View

Why we use excess of primers in PCR?

It is known that it prevent mispriming and unspecific amplification . can anyone explain the exact mechanism.

07 May 2023 1,153 6 View

DH5α competent cell contamination?

I streaked DH5 α culture in nalidixic and kanamycin plate to make sure free from contamination. I initiated three ml culture, then 30ml culture there also I added nalidixic acid. Then, I prepared...

14 August 2018 7,724 3 View

I need a help to develop the matlab coding for structural health monitoring.please kindly help me

Dear friends, i am doing the project in structural health monitoring. i need a help to develop and implement the coding matlab. please kindly help me. looking positive information

02 August 2017 5,493 9 View

Which blood collection method is ideal for 28 frequency in 7 days

We need to collect the blood samples 28 times in the period of one week. The required sample volume is 300 microlitre on each collection. We tried to collect the sample from tail vein. However, we...

02 August 2017 952 3 View

Ideal way to administer the powder diet into rat?

I need to feed the rat with powder high fat diet. We used to mix the powder high fat diet with coconut oil and make the powder diet into small balls. But, the rats are not eating these balls...

16 November 2016 6,512 5 View

How to compare the groups in which each group consists the data for two different duration?

There are four groups in our study and we measured the study parameters in two different time period in each group. So, there are two data for each group, for instance food intake from 01-30 days...

03 November 2016 6,356 1 View

Can any one suggest how to get satellite raw data at free off cost?

I have seen many websites where we can get free satellite date such as earth explorer. But all the images are modified with place name or latitude and longitude or post processed for some specific...

18 July 2016 1,493 4 View

What kind of methods I have to follow to give CCl4 on Rat through Intrapertonial (IP)?

Kindly suggest me the Dilution method and when I have to give? what is the time interval between drug and CCL4?

24 April 2016 5,097 8 View

Anyone can suggest me Where I can give my samle to check "In vitro hepatitis b surface antigen assay" in Maharashtra (Mumbai or Pune location)?

I have a my marine animal extract with me and I have to perform hepatitis b surface antigen in-vitro assay. Thank you.

17 March 2016 7,193 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can I apply boundary conditions in an orthotropic steel deck numerical model using ABAQUS software?

I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...

08 August 2024 719 0 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Unusual intensity drop in some sections of chromatograms in DDA?

Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...

07 August 2024 3,218 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View