Can anyone suggest me the list of free publishing journals in the field of microbiology?

I want to publish my research in a microbiology journal which publishes a research article without any charge. Could anyone suggest such journals?

01 February 2020 3,214 3 View

Could anyone let me know that after blocking with BSA , if we increase the concn. of detection antibody why it produce high background signals?

03 April 2015 3,868 3 View

Can anyone explain the difference between cutoff value and Limit of detection?

02 March 2015 1,610 4 View

Immunosesnor vs ELISA. Can any one let me know distinguish advantages of immunosensor over ELISA?

I have read a lot of articles and review articles regarding immunosensor, in the articles researcher compares the sensitivity of immunosensor vs ELISA. But other than this, I could not any...

31 December 2014 8,055 11 View

Can anyone let me know how to introduce the OH group on screen printed carbon electrodes?

I want to modified my screen printed electrode (SPCE) with OH group. Later, OH modified SPCE will be treated with APTES to introduce NH2 group on SPCE. Can anyone let me know a simple method to...

04 May 2014 429 3 View

Can any one let me know how to remove unnecessary protein from serum?

Actually when I perform my ELISA sandwich assay using commercially purchased antigen it works well but when I used positive serum sample it did not work. I assumed that serum protein interfered...

08 September 2013 9,839 6 View

How to remove inhibitors from serum samples?

When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...

07 August 2013 543 3 View

Which is the best hybridization buffer for dilution of antibodies and antigens in an ELISA based experiment?

I want to perform an ELISA test and I want to know what kind of buffer I should use to dissolve and dilute my antibody and antigen. The buffer for the antibody/antigen should enable them to...

01 February 2013 3,932 8 View

Can anyone let me know which chemical is suitable for the pre-treatment of carbon electrode?

According to literature there are so many chemicals like H2SO4, NaHCO3, HClO4,KMnO4 Saturated KCl and NaCO3 that can be use to activate the surface and even some author claimed that we can also...

08 September 2012 8,247 3 View

What concentration of antigens should be used the in ELISA? Should it be equal to the concentration of capturing antibodies or it should be higher?

In addition, can we use the 1.0mg/ml antibody for HRP-antibody conjugation? According to peroxidase labeling kit we should use 0.3ml @ 4.0mg/ml. However, this is too high of a concentration and it...

05 June 2012 9,369 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Benefits of the 24 welled plate?

We have always used the 96 welled plate, the 48 welled plate but apparently there is much evidence on the 24 welled plate and above all its benefits.It states it is the same you would use on ELISA...

28 February 2021 1,149 1 View

Standard curve shifting over time. How do I stabilise the ELISA?

24 February 2021 1,365 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

Surprisingly low antibody titers against SASRS-COV-2 RBD in hamsters compared to mice?

21 February 2021 6,958 2 View

Can anyone advise me about a cortisol antibody made in rabbit to set up an ELISA?

I am trying to set up a cortisol EIA using mouse anti-rabbit IGg as coating and a rabbit cortisol antibody as secondary ab. I am searching for a sensitive and specific cortisol Ab made in rabbit

20 February 2021 3,366 5 View