Om Prakash

12 Questions 14 Answers 0 Followers

Questions related from Om Prakash

Can anyone suggest me the list of free publishing journals in the field of microbiology?

I want to publish my research in a microbiology journal which publishes a research article without any charge. Could anyone suggest such journals?

02 February 2020 3,307 3 View

Could anyone let me know that after blocking with BSA , if we increase the concn. of detection antibody why it produce high background signals?

I want to know that why blocking is not effective when we increase the concentration of detection antibody. Is the blocking only effective for a certain range of antibody? or is there any other...

04 April 2015 3,993 3 View

Can anyone explain the difference between cutoff value and Limit of detection?

I am confused with LOD and cutoff value. Can anyone explain it? If possible, please share the different methods for finding the above mentioned value?

03 March 2015 2,161 4 View

Immunosesnor vs ELISA. Can any one let me know distinguish advantages of immunosensor over ELISA?

I have read a lot of articles and review articles regarding immunosensor, in the articles researcher compares the sensitivity of immunosensor vs ELISA. But other than this, I could not any...

01 January 2015 8,123 11 View

Can anyone let me know how to introduce the OH group on screen printed carbon electrodes?

I want to modified my screen printed electrode (SPCE) with OH group. Later, OH modified SPCE will be treated with APTES to introduce NH2 group on SPCE. Can anyone let me know a simple method to...

05 May 2014 499 3 View

Can any one let me know how to remove unnecessary protein from serum?

Actually when I perform my ELISA sandwich assay using commercially purchased antigen it works well but when I used positive serum sample it did not work. I assumed that serum protein interfered...

09 September 2013 9,942 6 View

How to remove inhibitors from serum samples?

When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...

08 August 2013 613 3 View

Which is the best hybridization buffer for dilution of antibodies and antigens in an ELISA based experiment?

I want to perform an ELISA test and I want to know what kind of buffer I should use to dissolve and dilute my antibody and antigen. The buffer for the antibody/antigen should enable them to...

02 February 2013 4,024 8 View

Can anyone let me know which chemical is suitable for the pre-treatment of carbon electrode?

According to literature there are so many chemicals like H2SO4, NaHCO3, HClO4,KMnO4 Saturated KCl and NaCO3 that can be use to activate the surface and even some author claimed that we can also...

09 September 2012 8,308 3 View

What concentration of antigens should be used the in ELISA? Should it be equal to the concentration of capturing antibodies or it should be higher?

In addition, can we use the 1.0mg/ml antibody for HRP-antibody conjugation? According to peroxidase labeling kit we should use 0.3ml @ 4.0mg/ml. However, this is too high of a concentration and it...

06 June 2012 9,451 4 View

Can we use the concentration of IgG below 3.8mg/ml for Peroxidase labeling Kit?

According to Roche company's protocol we have to use 3.8mg-4.2mg/ml IgG concentration for HRP-antibody conjugation. But the amount of antibody is too high to manage. Is it possible to reduce the...

06 June 2012 1,732 3 View

Can any one let me know the optimal concentration of capturing antibody and detection antibody in ELISA test?

Does it vary in different test kits?

06 June 2012 2,706 5 View