Could anyone let me know that after blocking with BSA , if we increase the concn. of detection antibody why it produce high background signals?

03 April 2015 3,868 3 View

Can anyone explain the difference between cutoff value and Limit of detection?

02 March 2015 1,610 4 View

Immunosesnor vs ELISA. Can any one let me know distinguish advantages of immunosensor over ELISA?

I have read a lot of articles and review articles regarding immunosensor, in the articles researcher compares the sensitivity of immunosensor vs ELISA. But other than this, I could not any...

31 December 2014 8,055 11 View

Can anyone let me know how to introduce the OH group on screen printed carbon electrodes?

I want to modified my screen printed electrode (SPCE) with OH group. Later, OH modified SPCE will be treated with APTES to introduce NH2 group on SPCE. Can anyone let me know a simple method to...

04 May 2014 429 3 View

Can any one let me know how to remove unnecessary protein from serum?

Actually when I perform my ELISA sandwich assay using commercially purchased antigen it works well but when I used positive serum sample it did not work. I assumed that serum protein interfered...

08 September 2013 9,839 6 View

How to remove inhibitors from serum samples?

When I perform immunoassay using a commercial antigen(purchased from abcam) it works but when I use a positive serum sample it does not work. Can any one let me know how to remove these inhibitors...

07 August 2013 543 3 View

Which is the best hybridization buffer for dilution of antibodies and antigens in an ELISA based experiment?

I want to perform an ELISA test and I want to know what kind of buffer I should use to dissolve and dilute my antibody and antigen. The buffer for the antibody/antigen should enable them to...

01 February 2013 3,932 8 View

Can anyone let me know which chemical is suitable for the pre-treatment of carbon electrode?

According to literature there are so many chemicals like H2SO4, NaHCO3, HClO4,KMnO4 Saturated KCl and NaCO3 that can be use to activate the surface and even some author claimed that we can also...

08 September 2012 8,247 3 View

What concentration of antigens should be used the in ELISA? Should it be equal to the concentration of capturing antibodies or it should be higher?

In addition, can we use the 1.0mg/ml antibody for HRP-antibody conjugation? According to peroxidase labeling kit we should use 0.3ml @ 4.0mg/ml. However, this is too high of a concentration and it...

05 June 2012 9,369 4 View

Can we use the concentration of IgG below 3.8mg/ml for Peroxidase labeling Kit?

According to Roche company's protocol we have to use 3.8mg-4.2mg/ml IgG concentration for HRP-antibody conjugation. But the amount of antibody is too high to manage. Is it possible to reduce the...

05 June 2012 1,657 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

Differences between sheep blood with Alsever solution vs citrated/defibrinated sheep blood in microbiological media?

I am aware of a study that has compared the use of citrated and defibrinated sheep's blood in microbiological media (Hair Sheep Blood, Citrated or Defibrinated, Fulfills All Req... ), but can...

10 February 2021 5,927 1 View

Hi everyone, I want to lower the pH of microbiological broth, what are the best options?

I will be using the lowered pH broth for MIC test of some disinfectants, and I do not want the acid to have any interfernece with MIC results.

03 February 2021 6,959 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

How do you quantify bacterial qPCR data?

Hi all, How would you use qPCR to quantify the amount of a particular bacterial species in a sample experimentally? I understand you could use specific primers for the 16s V region of a...

01 February 2021 7,153 4 View

How can I know the stationary phase of growth of several bacteria?

How can I know the stationary phase of growth of several bacteria? We are aiming to collect exoproteome of several bacteria. We know that the ideal time to collect exoproteome is during the...

31 January 2021 4,528 10 View