Andy Suh

2 Questions 3 Answers 0 Followers

Questions related from Andy Suh

Need better separation in Open SEC.

I am loading a total of 1mL of Bovine IgG and BSA (1mg/ml final concentration) into a 1cn diameter 12mL Sephadex G-75 superfine open column running at 15cm/hr. BIgG is ~150,000 MW while BSA is...

02 February 2013 1,866 1 View

SDS Gel Electrophoresis: I am looking to get sharper bands

I wanted 5uG in each lane. For Lane 2 I had a concentration of 1.08mg/mL = 1.08ug/uL. 5ug x 1uL/1.08ug = 4.63uL. I mixed (4.63uL x 3) with the same volume of laemlli sample buffer to get a total...

01 January 2013 1,726 8 View