I have prepared miRNA cDNA using Taqman advanced miRNA cDNA synthesis kit and used them with Taqman qPCR Assay. Now i want to assess expression level of more miRNAs in same samples using SYBR green chemistry. so is there any chance that it can be done using SYBR green with the same cDNA??
Dear Vikas,
If i can reformulate, you did extract miRNAs and proceeded to an RT reaction.What was the kit you use and what kind of taxman assay. If it was the advanced ones, you may be able to use your RT reaction with several advanced assays. If not, as the RT reaction was set with specific RT primers you won't be able to use different assays. I am not sure that you could do SYBR green based miRNAs PCR with Thermo-branded RT.
To use SYBR, I would recommend you to use Exiqon LNA primers and PCR reagents.
Hope this will help you.
Best.
Vikas Gaur
Yes you can.
Varkonyi-Gasic and Hellens, (2011) refer this for more info.
Dear Vikas,
You can use the same Taqman primer for SYBR green based quantification.
Dear Sankara Naynar my question is about miRNA cDNA and their use in Real time with SYBR green, not about the Taqman Primers.
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