Vikas Gaur

3 Questions 3 Answers 0 Followers

Questions related from Vikas Gaur

Can we use miRNA cDNA, prepared using Taqman advanced miRNA cDNA synthesis kit with SYBR green assay?

I have prepared miRNA cDNA using Taqman advanced miRNA cDNA synthesis kit and used them with Taqman qPCR Assay. Now i want to assess expression level of more miRNAs in same samples using SYBR...

06 June 2019 6,918 6 View

MiRNA target gene prediction?

I want to predict target genes for some miRNAs in my disease of interest. i checked online i got a bunch of genes (more than 1000) for a every single miRNA. Can anyone please suggest me that how...

06 June 2018 4,411 0 View

I want to do miRNA expression analysis from total cDNA. please suggest ?

i have isolated total RNA from PBMCs and then synthesized cDNA from this total RNA. Now i want to check the miRNA expression in these cDNA with TaQman probes. please suggest. Is there is any...

11 November 2017 9,464 3 View