Can we use M13 Reverse and M13 Forward (generally used for sequencing insert in pUC18 MCS) for seuencing/amplifying fragment of M13mp18?

07 July 2014 1 7K Report

Do these two primers have a sequence in M13mp18 DNA, as the name suggests that these are from M13? Please provide an explanation.

Amanda O'Donnell

Why don't you retrieve the sequence for M13mp18 from NCBI and search for the particular sequence of primer that you have there.

Badges
Science method

More Ravinder Kumar's questions See All

Do plasmid DNA also get extracted alongwith genomic DNA during the genomic DNA extraction,?

Is there plasmid DNA contamination in genomic DNA preparations?

06 July 2015 6,044 0 View

Why E. coli BL21 strain can not be used for plasmid preps?

01 February 2015 9,676 0 View

Is there any chemical difference in Heparin resin used in HiTrap Heparin HP and Heparin sepharose 6 FF from GE healthcare?

Hitrap Heparin HP is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method and in case of Heparin sepharose 6 FF, Heparin is linked to the Sepharose...

31 December 2014 6,667 1 View

Can we sequence DNA on sequencing gels using fluorescent labels?

Can fluorescent labels be detected with a phosphor imager? We have Fujifilm- FLA 9000 and Biorad Screen Eraser-K set up for detection.

10 November 2014 8,882 3 View

How can I remove DNA from bacterial crude lysates?

Cells are lysed by sonication and further I want to remove DNA from lysates? What is the best method without damaging/reducing protein/enzyme activities?

06 July 2014 791 12 View

Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

02 March 2014 7,511 1 View

How do we equilibrate the millipore protein concentrator while using it for the first time?

Can it be reused, and if yes, how many times?

01 February 2014 5,324 8 View

Can coomassie stained gels which have been destained, be stained again? With coomassie stain and silver stain?

I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...

01 February 2014 8,467 12 View

Do proteins retain activity after acetone precipitation?

I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.

01 February 2014 9,575 5 View

What is the binding capacity of DEAE cellulose?

Concentration of sample is 10-12 mg/ml and I am planning to use 4 ml DEAE cellulose resin. I need to know the amount of protein sample required to be loaded on the column to get good resolution?

01 February 2014 5,018 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View