Do plasmid DNA also get extracted alongwith genomic DNA during the genomic DNA extraction,?

Is there plasmid DNA contamination in genomic DNA preparations?

06 July 2015 5,942 0 View

Why E. coli BL21 strain can not be used for plasmid preps?

Why E. coli BL21 strain can not be used for plasmid preps?

01 February 2015 9,576 0 View

Is there any chemical difference in Heparin resin used in HiTrap Heparin HP and Heparin sepharose 6 FF from GE healthcare?

Hitrap Heparin HP is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method and in case of Heparin sepharose 6 FF, Heparin is linked to the Sepharose...

31 December 2014 6,439 1 View

Can we sequence DNA on sequencing gels using fluorescent labels?

Can fluorescent labels be detected with a phosphor imager? We have Fujifilm- FLA 9000 and Biorad Screen Eraser-K set up for detection.

10 November 2014 8,774 3 View

How can I remove DNA from bacterial crude lysates?

06 July 2014 698 12 View

Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

02 March 2014 7,422 1 View

How do we equilibrate the millipore protein concentrator while using it for the first time?

Can it be reused, and if yes, how many times?

01 February 2014 5,205 8 View

Can coomassie stained gels which have been destained, be stained again? With coomassie stain and silver stain?

I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...

01 February 2014 8,373 12 View

Do proteins retain activity after acetone precipitation?

I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.

01 February 2014 9,457 5 View

What is the binding capacity of DEAE cellulose?

Concentration of sample is 10-12 mg/ml and I am planning to use 4 ml DEAE cellulose resin. I need to know the amount of protein sample required to be loaded on the column to get good resolution?

01 February 2014 4,914 5 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View