Do plasmid DNA also get extracted alongwith genomic DNA during the genomic DNA extraction,?

Is there plasmid DNA contamination in genomic DNA preparations?

06 July 2015 5,942 0 View

Why E. coli BL21 strain can not be used for plasmid preps?

Why E. coli BL21 strain can not be used for plasmid preps?

01 February 2015 9,576 0 View

Is there any chemical difference in Heparin resin used in HiTrap Heparin HP and Heparin sepharose 6 FF from GE healthcare?

Hitrap Heparin HP is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method and in case of Heparin sepharose 6 FF, Heparin is linked to the Sepharose...

31 December 2014 6,439 1 View

Can we use M13 Reverse and M13 Forward (generally used for sequencing insert in pUC18 MCS) for seuencing/amplifying fragment of M13mp18?

Do these two primers have a sequence in M13mp18 DNA, as the name suggests that these are from M13? Please provide an explanation.

06 July 2014 6,939 1 View

How can I remove DNA from bacterial crude lysates?

06 July 2014 698 12 View

Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

02 March 2014 7,422 1 View

How do we equilibrate the millipore protein concentrator while using it for the first time?

Can it be reused, and if yes, how many times?

01 February 2014 5,205 8 View

Can coomassie stained gels which have been destained, be stained again? With coomassie stain and silver stain?

I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...

01 February 2014 8,373 12 View

Do proteins retain activity after acetone precipitation?

I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.

01 February 2014 9,457 5 View

What is the binding capacity of DEAE cellulose?

Concentration of sample is 10-12 mg/ml and I am planning to use 4 ml DEAE cellulose resin. I need to know the amount of protein sample required to be loaded on the column to get good resolution?

01 February 2014 4,914 5 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View