Why E. coli BL21 strain can not be used for plasmid preps?
Is there plasmid DNA contamination in genomic DNA preparations?
06 July 2015 6,044 0 View
Hitrap Heparin HP is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method and in case of Heparin sepharose 6 FF, Heparin is linked to the Sepharose...
31 December 2014 6,667 1 View
Can fluorescent labels be detected with a phosphor imager? We have Fujifilm- FLA 9000 and Biorad Screen Eraser-K set up for detection.
10 November 2014 8,882 3 View
Do these two primers have a sequence in M13mp18 DNA, as the name suggests that these are from M13? Please provide an explanation.
06 July 2014 7,047 1 View
Cells are lysed by sonication and further I want to remove DNA from lysates? What is the best method without damaging/reducing protein/enzyme activities?
06 July 2014 791 12 View
As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?
02 March 2014 7,511 1 View
Can it be reused, and if yes, how many times?
01 February 2014 5,324 8 View
I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...
01 February 2014 8,467 12 View
I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.
01 February 2014 9,575 5 View
Concentration of sample is 10-12 mg/ml and I am planning to use 4 ml DEAE cellulose resin. I need to know the amount of protein sample required to be loaded on the column to get good resolution?
01 February 2014 5,018 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View
I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...
28 July 2024 7,559 1 View
Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...
25 July 2024 2,532 3 View
I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...
23 July 2024 5,296 0 View
