Ravinder Kumar

14 Questions 12 Answers 0 Followers

Questions related from Ravinder Kumar

Do plasmid DNA also get extracted alongwith genomic DNA during the genomic DNA extraction,?

Is there plasmid DNA contamination in genomic DNA preparations?

07 July 2015 6,008 0 View

Why E. coli BL21 strain can not be used for plasmid preps?

02 February 2015 9,630 0 View

Is there any chemical difference in Heparin resin used in HiTrap Heparin HP and Heparin sepharose 6 FF from GE healthcare?

Hitrap Heparin HP is coupled to the Sepharose High Performance base matrix via the N-hydroxysuccinamide coupling method and in case of Heparin sepharose 6 FF, Heparin is linked to the Sepharose...

01 January 2015 6,591 1 View

Can we sequence DNA on sequencing gels using fluorescent labels?

Can fluorescent labels be detected with a phosphor imager? We have Fujifilm- FLA 9000 and Biorad Screen Eraser-K set up for detection.

11 November 2014 8,833 3 View

Can we use M13 Reverse and M13 Forward (generally used for sequencing insert in pUC18 MCS) for seuencing/amplifying fragment of M13mp18?

Do these two primers have a sequence in M13mp18 DNA, as the name suggests that these are from M13? Please provide an explanation.

07 July 2014 7,003 1 View

How can I remove DNA from bacterial crude lysates?

Cells are lysed by sonication and further I want to remove DNA from lysates? What is the best method without damaging/reducing protein/enzyme activities?

07 July 2014 756 12 View

Does anybody know of a plasmid or DNA vector which lacks or has a single TTAA site?

As it is a 4 bp sequence, it frequently occurs in most plasmids. If anybody knows of such plasmid lacking or having single TTAA site?

03 March 2014 7,475 1 View

What is the binding capacity of DEAE cellulose?

Concentration of sample is 10-12 mg/ml and I am planning to use 4 ml DEAE cellulose resin. I need to know the amount of protein sample required to be loaded on the column to get good resolution?

02 February 2014 4,981 5 View

How do we equilibrate the millipore protein concentrator while using it for the first time?

Can it be reused, and if yes, how many times?

02 February 2014 5,277 8 View

How can we elute protein bands from native PAGE so as to check activity?

I want to elute active protein from native gel and still retain activity. Can it be done? Any reference would be helpful.

02 February 2014 1,391 10 View

Can coomassie stained gels which have been destained, be stained again? With coomassie stain and silver stain?

I have stained my SDS-PAGE with coomassie blue but bands are very light. Probably, the stain was not good. Can the gel be stained again with new coomassie or silver stain? Will the staining be...

02 February 2014 8,435 12 View

Do proteins retain activity after acetone precipitation?

I need to concentrate my protein samples for running SDS-PAGE as well as checking activity.

02 February 2014 9,530 5 View

Can DNA fragments which have been precipitated with ethanol or isopropanol be ligated?

The DNA fragments have been previously digested with restriction enzymes, will ligation after ethanol precipitation work?

01 January 2014 3,496 9 View

What is the minimum amount of protein that can be easily visualised on PAGE by coomassie blue staining and silver staining?

I have a purified enzyme which is giving good activity on its substrate but I am not able to visualise any bands on SDS-PAGE gels. How much is the minimum of protein am I need to load?

01 January 2014 3,694 19 View