I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice with active drug?
I would like to ask, which type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis. Currently, my extraction method involves mixing mice plasma with saline,...
04 May 2018 1,058 6 View
I would like to know which brand and what type of activated charcoal or activated carbon (lab grade) that I should use to purify plasma samples from impurities or endogenous compound for HPLC use....
02 March 2018 5,567 0 View
I dont quite understand the following and would like to clarify the meaning: 1. For Lower Limit of Quantification (LLOQ), the analyte response at the LLOQ should be at least 5 times the response...
07 August 2017 6,934 5 View
I am going to do a biodistribution studies on tocotrienol in vivo. Previously my tocotrienol was tagged with a fluorescent probe. However, I would like to change my approach and using a...
03 April 2017 4,352 0 View
If i have three concentration (20ug/ml, 40ug/ml and 100ug/ml). Each of this concentration give different excitation wavelength (e.g 273, 275 and 276nm respectively) and different fluorescent...
10 November 2016 3,701 0 View
I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...
10 November 2016 7,921 3 View
I would like to know why we need to include a healthy animal group? For example: If I have 3 groups: 1st group is a tumour induced mice treated with a non-targeted drug. 2nd group is a tumour...
05 June 2016 5,628 8 View
I am trying to see whether I can characterise my fluorescence labelled drug excitation, emission wavelength using either spectrofluorometer and fluorescence spectrometer.
05 June 2016 2,629 0 View
A powder extract is dissolved in pure DMSO first and diluted with sterile saline (0.9% NaCl) at 50 mg/ml with the final DMSO concentration at 10% Does this mean that the poweder of 50mg is...
06 July 2015 4,583 0 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
How to determine tannin content in fodder pellets?
22 July 2024 8,163 3 View
I'm trying to use cassowary footprint to estimate their population since their footprints are distinguishable from other animal on the field. is it really possible to do that? since it's really...
18 July 2024 3,729 7 View
Today I took this video in Southern Bavaria, Germany. Video: https://www.youtube.com/watch?v=cCy1eLM6aE0 A female Maculinea rebeli was continuously tapping the leaves of Picea abies with its...
15 July 2024 8,099 4 View
I am looking for an in-vivo model to evaluate standardized herbal extract for its potential as a mast cell stabilizer.
14 July 2024 779 0 View
I need help for find a subject for PhD in insect physiology
12 July 2024 8,227 1 View
In My article: The Extended Pedersen hypothesis (published, February 1988 Clinical Physiology and Biochemistry 6(2):68-73,) there is mistake of the name of first Author: it is not Catherine...
11 July 2024 1,801 2 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View