11 November 2016 3 8K Report

I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters:

Excitation: 273-276nm

Slit: 20

Smoothing: 40

As my concentration increases from 20ug/ml -> 100ug/ml, the intensity decreases. 

Could anyone explain this phenomenon? As I will need to do a standard curve. 

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