Fu Shun Wong

10 Questions 2 Answers 0 Followers

Questions related from Fu Shun Wong

What type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis?

I would like to ask, which type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis. Currently, my extraction method involves mixing mice plasma with saline,...

05 May 2018 1,014 6 View

What type of activated charcoal can be used to purify plasma samples?

I would like to know which brand and what type of activated charcoal or activated carbon (lab grade) that I should use to purify plasma samples from impurities or endogenous compound for HPLC use....

03 March 2018 5,512 0 View

Help with explanation for HPLC validation protocol

I dont quite understand the following and would like to clarify the meaning: 1. For Lower Limit of Quantification (LLOQ), the analyte response at the LLOQ should be at least 5 times the response...

08 August 2017 6,894 5 View

Are there any biodistribution studies using fluorescent cholesterol probe?

I am going to do a biodistribution studies on tocotrienol in vivo. Previously my tocotrienol was tagged with a fluorescent probe. However, I would like to change my approach and using a...

04 April 2017 4,301 0 View

Would different concentration at same excitation influence fluorescence intensity?

If i have three concentration (20ug/ml, 40ug/ml and 100ug/ml). Each of this concentration give different excitation wavelength (e.g 273, 275 and 276nm respectively) and different fluorescent...

11 November 2016 3,657 0 View

Why does intensity drops as concentration increases?

I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...

11 November 2016 7,871 3 View

Can we use clean mice plasma for pharmacokinetic calibration curve?

I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice...

10 October 2016 8,905 0 View

What is the difference between spectrofluorometer, fluorescence spectrometer and fluorescence spectrophotometer?

I am trying to see whether I can characterise my fluorescence labelled drug excitation, emission wavelength using either spectrofluorometer and fluorescence spectrometer.

06 June 2016 2,581 0 View

Why do we include a healthy group for invivo study (animal model)?

I would like to know why we need to include a healthy animal group? For example: If I have 3 groups: 1st group is a tumour induced mice treated with a non-targeted drug. 2nd group is a tumour...

06 June 2016 5,546 8 View

Is this explanation correct?

A powder extract is dissolved in pure DMSO first and diluted with sterile saline (0.9% NaCl) at 50 mg/ml with the final DMSO concentration at 10% Does this mean that the poweder of 50mg is...

07 July 2015 4,539 0 View