What type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis?

I would like to ask, which type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis. Currently, my extraction method involves mixing mice plasma with saline,...

04 May 2018 935 6 View

What type of activated charcoal can be used to purify plasma samples?

I would like to know which brand and what type of activated charcoal or activated carbon (lab grade) that I should use to purify plasma samples from impurities or endogenous compound for HPLC use....

02 March 2018 5,464 0 View

Help with explanation for HPLC validation protocol

I dont quite understand the following and would like to clarify the meaning: 1. For Lower Limit of Quantification (LLOQ), the analyte response at the LLOQ should be at least 5 times the response...

07 August 2017 6,856 5 View

Are there any biodistribution studies using fluorescent cholesterol probe?

I am going to do a biodistribution studies on tocotrienol in vivo. Previously my tocotrienol was tagged with a fluorescent probe. However, I would like to change my approach and using a...

03 April 2017 4,249 0 View

Why does intensity drops as concentration increases?

I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...

10 November 2016 7,791 3 View

Would different concentration at same excitation influence fluorescence intensity?

10 November 2016 3,607 0 View

Can we use clean mice plasma for pharmacokinetic calibration curve?

I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice...

09 October 2016 8,826 0 View

Why do we include a healthy group for invivo study (animal model)?

05 June 2016 5,439 8 View

What is the difference between spectrofluorometer, fluorescence spectrometer and fluorescence spectrophotometer?

05 June 2016 2,520 0 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Timeout error!! Processor in the loop (PIL) using STM32F4 Matlab 2018a?

Dear Researchers I am trying to perform a PIL simulation using STM32F4 Discovery board and comunication serial USB TO TTL. During simulation I receive the following timeout error: An error...

01 March 2021 2,327 1 View

Bought HUVEC from Invitrgen.need to revive it.expected it is cryofreezed in DMSO.Do I need to remove the DMSO for plating the cell for the 1st time?

After I cryofreeze the cell do I need to remove the DMSO for culturing the cell for next passages .

28 February 2021 7,738 3 View

POlymer+DMSO solution and moisture??

What could be the reason of moisture at the sides of Vial when I am preparing my polmyer+DMSO solution for membrane fabrication? What etiquettes I am possibly missing here?

28 February 2021 6,516 5 View

No title

I am trying to find how to know the density needed for a pva electrospinning in a 0.6mm neddle, the electrospinning will be from top to bottom and the problem I really have is the solution because...

25 February 2021 2,615 3 View

How do I choose contrasts in SPM8?

I am learning how to use SPM for PET and PET-MR analysis, but I haven't found any great resources that explain how contrasts work. I have a series of PET images where our hypothesis argues that...

24 February 2021 1,534 2 View

How Can I clain a removal of a co-author?

I would like to make a correction in a co-author in your paper which is wrong. This name is also in other papers of Kenton Ko. Zey Ko is not a Co-author . The correct name is Zdenka...

24 February 2021 3,476 4 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View