I am trying to see whether I can characterise my fluorescence labelled drug excitation, emission wavelength using either spectrofluorometer and fluorescence spectrometer.
I would like to ask, which type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis. Currently, my extraction method involves mixing mice plasma with saline,...
04 May 2018 1,058 6 View
I would like to know which brand and what type of activated charcoal or activated carbon (lab grade) that I should use to purify plasma samples from impurities or endogenous compound for HPLC use....
02 March 2018 5,567 0 View
I dont quite understand the following and would like to clarify the meaning: 1. For Lower Limit of Quantification (LLOQ), the analyte response at the LLOQ should be at least 5 times the response...
07 August 2017 6,934 5 View
I am going to do a biodistribution studies on tocotrienol in vivo. Previously my tocotrienol was tagged with a fluorescent probe. However, I would like to change my approach and using a...
03 April 2017 4,352 0 View
If i have three concentration (20ug/ml, 40ug/ml and 100ug/ml). Each of this concentration give different excitation wavelength (e.g 273, 275 and 276nm respectively) and different fluorescent...
10 November 2016 3,701 0 View
I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...
10 November 2016 7,921 3 View
I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice...
09 October 2016 8,959 0 View
I would like to know why we need to include a healthy animal group? For example: If I have 3 groups: 1st group is a tumour induced mice treated with a non-targeted drug. 2nd group is a tumour...
05 June 2016 5,628 8 View
A powder extract is dissolved in pure DMSO first and diluted with sterile saline (0.9% NaCl) at 50 mg/ml with the final DMSO concentration at 10% Does this mean that the poweder of 50mg is...
06 July 2015 4,583 0 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
Dear All: I have to quantify the fluorescence intensity of cells that are fluorescent to two different markers, so i'm interested in finding out if this is possible. Previously I have...
22 July 2024 8,770 1 View