I would like to know which brand and what type of activated charcoal or activated carbon (lab grade) that I should use to purify plasma samples from impurities or endogenous compound for HPLC use.
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I would like to ask, which type of syringe filter is suitable to purify extracted plasma samples for HPLC Analysis. Currently, my extraction method involves mixing mice plasma with saline,...
04 May 2018 1,058 6 View
I dont quite understand the following and would like to clarify the meaning: 1. For Lower Limit of Quantification (LLOQ), the analyte response at the LLOQ should be at least 5 times the response...
07 August 2017 6,934 5 View
I am going to do a biodistribution studies on tocotrienol in vivo. Previously my tocotrienol was tagged with a fluorescent probe. However, I would like to change my approach and using a...
03 April 2017 4,352 0 View
If i have three concentration (20ug/ml, 40ug/ml and 100ug/ml). Each of this concentration give different excitation wavelength (e.g 273, 275 and 276nm respectively) and different fluorescent...
10 November 2016 3,701 0 View
I am currently doing a fluorescence intensity scan with my compound. I am using the following parameters: Excitation: 273-276nm Slit: 20 Smoothing: 40 As my concentration increases from 20ug/ml...
10 November 2016 7,921 3 View
I would like to know if there is any publication that supports the use of clean mouse plasma (eg. from sigma) to use it as a support for the calibration curve when compared to the PK of a mice...
09 October 2016 8,959 0 View
I would like to know why we need to include a healthy animal group? For example: If I have 3 groups: 1st group is a tumour induced mice treated with a non-targeted drug. 2nd group is a tumour...
05 June 2016 5,628 8 View
I am trying to see whether I can characterise my fluorescence labelled drug excitation, emission wavelength using either spectrofluorometer and fluorescence spectrometer.
05 June 2016 2,629 0 View
A powder extract is dissolved in pure DMSO first and diluted with sterile saline (0.9% NaCl) at 50 mg/ml with the final DMSO concentration at 10% Does this mean that the poweder of 50mg is...
06 July 2015 4,583 0 View
I tried four trials of the same Copper Phosphides sample in Alkaline medium ( 0.5M KOH) with Hg/HgO reference electrode and Pt as counter electrode. I used 0.001 V/s scan rate for first three...
10 August 2024 3,629 1 View
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?
09 August 2024 3,291 2 View
I have been getting a low plasma and no coating while doing rf sputtering of copper doped ZnS using a power of 140 watts and at pressure of 6.5x10-3 Mb.
07 August 2024 4,526 4 View
Global ‘Weather’ has stopped, as ‘aqua thermionic instituted by the ‘Global thermo atmospherics has instituted global thermodynamics of 120º C + and thus all waters are, or have boiled into a...
06 August 2024 8,716 0 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
The starting material is a finely ground powder. Some particles contain more graphene than others.
30 July 2024 3,978 2 View
I am currently working on modeling FCC catalyst regeneration and have come across a point of uncertainty that I hope you can assist me with. In my previous models, I have utilized lumped kinetics...
30 July 2024 6,137 0 View
A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids
28 July 2024 4,985 1 View