Can we measure tumour volume or area from H&E stained slides?

I have H&E stained slides of lungs from mouse models of lung cancer. I was wondering if I can calculate tumour volume or area from the H&E stained slides. Thank you. Vrushali

08 September 2017 1,014 1 View

What are the parameters for selecting a transcription factor bound to promoter region when using PROMO:Alggen?

I am interested in finding transcription factors binding to promoter of a virus, Merkel cell polyomavirus. I got several factors with varying dissimilarity. Is there any criteria that can be used...

04 May 2016 632 2 View

How do I find promoter sequence of a viral genome ?

I want to align promoter sequence of a small T antigen and large T antigen of virus (merkel cell polyomavirus). I am aware that for human genome we generally subtract and add 1 kb from genome to...

09 October 2015 8,022 1 View

How to choose a journal to publish your paper?

I have some data from my MSc dissertation which I am thinking of publishing but I am not sure how to publish a paper eg how to choose a journal, impact factor etc. Can anyone provide...

01 February 2015 484 6 View

How much of Bioinformatics knowledge do I need to elucidate the functional effect of missense mutation?

I am a postgraduate student and have completed MSc in Medical genetics. I intend to apply for PhD in future and my current interest lies in analysing missense mutations for their pathogenecity. I...

31 December 2014 7,138 3 View

Does anyone have experience in generating workflow in CLC Genomics Workbench 7.5?

Hello everyone, I am supposed to use CLC Genomics workbench to perform trio analysis to find candidate gene responsible for rare disorder. Can anyone help me in generating Workflow in CLC Genomics?

09 October 2014 8,053 2 View

How can one perform trio analysis using CLC genomics workbench 7.5?

I have a exome sequencing data of parents and their child. We are trying to identify the candidate genes responsible for some abnormality.I have never used CLC genomics workbench. Do I need a...

09 October 2014 3,310 4 View

Computing h bonds in swiss pdb viewer?

I wanted to see the h bond interaction within a protein so I used swiss pdb viewer. Generally it gives only green dotted line as h bonds, but it also gave purple colored dotted line. Can anyone...

07 August 2014 1,714 1 View

Is there any criteria to choose a chaperon to crystallize a intrinsically disordered region in a protein?

I read an article where an intrinsically flexible protein was crystallized using chaperon (here chaperon is a protein that helps to stabilize other protein) . i was wondering what would be the...

07 August 2014 7,548 5 View

For using Align GVGD we have to paste MSAs, so how does one choose the number of sequences that share a similarity between the gene of interest?

I am trying to analyse APC gene. I used blast to get multiple sequence alignment. How many sequences should I choose to input in Align GVGD. Is there any criteria?

06 July 2014 6,001 1 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View