What is the thumb rule for quality image in Confocal microscopy?

Dear researchers, I'm working with FITC and Rhodamine B dyes and I use Sequential scanning. I will adjust the image quality through Smart Gain and Smart Offset and maintain QLUT in reddish...

08 April 2019 9,057 3 View

Size Exclusion Chromatography, which software to use?

Hi Researchers, Greetings!! I like to determine molecular weight of the starch through SEC. I'm following the attached manuscript (Hasjim et al. 2010, Biomacromolecules, 11, 3600-3608). I have a...

04 February 2019 8,859 3 View

Where can I get Tutorial for working with Fluent's Population balance model?

I like to work with PBE coupled with CFD using Fluent solver. I couldn't find any working tutorials in the web other than a pdf file made in 2012. From the Fluent forum, I understand it was...

03 December 2018 2,461 6 View

Is it possible IGF1R can interact with CHIP chaperone?I have read some papers its confused me so i want to know is it possible?

MOLECULAR BIOLOGY, IGF1R AND CHIP CHAPERONE

21 May 2018 5,124 3 View

How to improve the chilling tolerance of tomato?

I am looking for answers from management aspects of improving tolerance. Not by genetic or plant breeding approach?

23 October 2016 7,693 5 View

Why does the unaligned Nematic liquid crystal sample give Diffuse rings but the aligned one give Arcs in 2D X ray pattern?

Field : Liquid crystal

12 November 2014 7,304 5 View

How can I improve Iron (Fe) and Zinc (Zn) nutrition in millets?

Through agronomical, cultural, physiological, genetical or molecular approaches?

09 April 2014 6,014 4 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Which polarizers are usually used to measure the birefringence of zebrafish muscles?

I already found several publications on that topic, however, the specifications for the used filters are often missing. Some more specific information than "fish is placed between to polarizing...

23 February 2021 1,934 1 View

How to calculate the Atomic Polar Tensor using DFT ?

Dear friends, I am a beginner at DFT calculation. I have done a structure optimization using DFT- B3LYP/6-31G*, Nevertheless, I don't know How to calculate the Atomic Polar Tensor (APT) in...

17 February 2021 9,082 3 View

How to obtain free lipids starting from concentration over the CMC?

I need to work with free lipids at increasing concentration (50%, 60% of the CMC ect.). I'm now working with POPC and POPG. To reach these concentrations I have to start from concentration over...

17 February 2021 1,676 3 View

Solid Phase Extraction of drug product containing nanoparticles ?

Hi Everyone, I am trying to develop an SPE method for the vaccine which contains Matrix-M (saponin Quillaja Saponaria ) which is in nano-particle form and size is around 40nm. These nanoparticles...

16 February 2021 1,389 2 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View