Is it possible IGF1R can interact with CHIP chaperone?I have read some papers its confused me so i want to know is it possible?

More Parthasarathi Barik's questions See All

What possible issue am I facing in this Alchemical FEP calculations using NAMD?

I am trying to run my tcl script for NAMD. Running separate MD simulation for each forward and backward runs. Forward run is fine however backward.inp abruptly terminates showing output only till...

17 June 2024 861 1 View

Why there are so many holes in brain cryostat sections?

whenever I take cryostat sections of rat brain, many holes are distorting the normal morphology(image attached). what may be the reason? protocol which we follow: * Perfuse the brain in PBS and...

15 June 2024 1,282 1 View

How to get rid of bacterial contamination during bacmid transfection to sf-21 cells?

Hello all, I am trying to transfect my bacmid to sf-21 cell line for baculovirus generation and I am facing a bacterial contamination in the transfected well. I used alkaline lysis method to...

15 May 2024 3,554 1 View

What is meant by the protein crystal structures obtained from uncultured bacterium on RCSF:PDB site?

While looking for my protein of interest, I came across PDB structures obtained from uncultured bacterium. How are these crystal structures different and what is the credibility of using these...

23 August 2023 5,325 0 View

Has Fusarium sacchari reported causing leaf blotch of orchid in "India" ?

First report of Fusarium causing leaf blotch of orchid in "India"?

22 August 2023 7,007 0 View

Please help anyone for identification of hymenopteran parasitoids of Callosobruchus chinensis?

Please help anyone for identification of hymenopteran parasitoids of Callosobruchus chinensis.

15 August 2023 5,481 1 View

Can anyone explain the "'step's and Load" part of Lateral dynamic load analysis of monopile foundation in ABAQUS ?

Suppose, I am having a soil-pile system. I want to apply a lateral load (Sinusoidal) on to the pile top and simulate the model. Kindly anyone explain the 'steps and load' part in abaqus.

28 February 2023 5,327 0 View

Sir, how can we draw a 3D surface by rotating an analytical curve about X-axis in CST MW Studio?

The equation of the analytical curve is :u(t)=t & v(t)= sqrt((R^2)-(t^2))-B; where R=2.125 & B=1.875, t=0 to 1. I was able to draw the analytical curve..but wasn't able to rotate it about...

15 December 2022 8,293 0 View

How to measure mean annual and mean seasonal seasonal discharge from daily data?

I have daily discharge data in the period between 1950 and 2010. How can I calculate "mean annual" and "mean seasonal" discharge from the daily data.

11 July 2022 1,672 0 View

Issue with MC3T3-E1 differentiation cell culture methods . Any advice?

I'm having an issue with differentiaon media (DM) of MC3T3-E1 Subclone 4 cells I am following the ATCC recommendations for media and supplements (alpha-MEM + 10% FBS + 1X anti/anti...

05 September 2021 5,951 5 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Sagar Bashyal

Some paper suggest

CHIP( C-terminus of Hsp70-interacting protein) is not able to ubiquitylate the insulin-like growth factor receptor 1 (IGF1R) . Article The Ubiquitin Ligase CHIP Integrates Proteostasis and Aging ...

Parthasarathi Barik

Thank you so much Sagar, I also want to know, whether this mechanism is same in all different types of cells?

Stuart Maudsley

Here's another interesting article for you ! - http://cancerres.aacrjournals.org/content/canres/early/2018/05/05/0008-5472.CAN-17-3498.full.pdf