Vishnu Rompicharla

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Questions related from Vishnu Rompicharla

What are the ideal reaction conditions for conjugation/coupling reactions of PAMAM dendrimers? DMF/DMSO or Aqueous buffers?

For example, to couple amine group of G4 PAMAM dendrimer with acid group of a small molecule, can a mixture of buffer and/or DMF be used? What are the best possible reaction conditions for such...

11 January 2016 3,296 2 View

What could be the reason for low entrapment efficiency of a lipophilic drug in PLGA nanoparticles?

I have used PLGA (50:50) of different molecular weight ranges (low-high) to entrap a lipophilic drug by nanoprecipitation method. The %EE obtained by HPLC analysis shows nothing has been...

23 March 2015 6,452 7 View

What is the best method to separate unentrapped hydrophobic drug from a liposome formulation?

To find the amount of drug entrapped in a liposome, which is the best method! Dialysis or centrifugation or any other? also, please specify how we could be sure to discriminate the amount of...

20 January 2015 1,744 51 View

Why are functionalized lipids supplied as sodium/ammonium salts?

For example, lipids like DSPE, DMPC, DOPE are supplied as ammonium salt/sodium salt. Is it for the stability or for any specific reason?

05 January 2015 6,768 2 View