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Questions related from Vishnu Rompicharla
For example, to couple amine group of G4 PAMAM dendrimer with acid group of a small molecule, can a mixture of buffer and/or DMF be used? What are the best possible reaction conditions for such...
11 January 2016 3,379 2 View
We are working on synthesis of Paclitaxel modified as NHS ester at 2' position to further couple it to an amine. PTX is initially converted to PTX-hemisuccinate using succinic anhydride and which...
03 September 2015 7,937 1 View
We are working on nanoparticle systems and conjugation. Kindly suggest who can support by providing free samples of mPEG amine or PLGA-PEG copolymer. Thanks
27 April 2015 4,684 1 View
I have used PLGA (50:50) of different molecular weight ranges (low-high) to entrap a lipophilic drug by nanoprecipitation method. The %EE obtained by HPLC analysis shows nothing has been...
23 March 2015 6,518 7 View
I have tried synthesizing PEG-PLGA diblock copolymer using traditional EDC/NHS reaction. The problem here is the yield of the final product. It was around 20%. What could be the reason for this?...
26 February 2015 7,552 7 View
We wish to prepare chitosan nanoparticles of a class IV drug. What molecular weight of chitosan should I use to get considerable size and encapsulation. Also advice on deacetylation degree.
20 February 2015 4,009 2 View
To find the amount of drug entrapped in a liposome, which is the best method! Dialysis or centrifugation or any other? also, please specify how we could be sure to discriminate the amount of...
20 January 2015 1,805 51 View
It is possible that the micropipette tips/eppendorf tubes/plastic vials may interact with the chloroform solution of lipids like PC which may cause stability issues? How do you transfer or handle...
20 January 2015 2,413 1 View
For example, lipids like DSPE, DMPC, DOPE are supplied as ammonium salt/sodium salt. Is it for the stability or for any specific reason?
05 January 2015 6,824 2 View
