15 July 2019 1 3K Report

I wanted to know if primary culture of neuronal cells could be cryopreserved and if the said protocol can ensure any cell revival after thawing the cryopreserved culture

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Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar  in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

Can I use zwitterionic buffers to set pH (5, 6, 7, 8, 9) of the EPA test solutions?If so, how?

I am working on a toxicity test and I want to set the pH of EPA medium to 5, 6, 7, 8, 9. I am planning to use zwitterionic buffers in powder form (MES, MOPS, etc.) to set these specific pH's as...

17 August 2023 485 0 View

How to convert particles/km2 of microplastics concentration in water to particles/m3?

I want to convert the unit of microplastics concentration in water from particles/km2 to particles/m3 and particles/L to particles/m3. Is there any formula or what are the conversion steps?

30 June 2023 7,345 4 View

How to calculate the Energy consumption (kWh) of electric pump used during the irrigation process?

Want to calculate the electric pump energy

18 February 2023 8,883 7 View

Is there any Cloudsim dataset for microservices workload?

i want workload for microservices consisting of CPU, Memory and BW traces to use as dataset for scheduling cloudlets on container

10 February 2023 8,336 0 View

Is their any alternative method for CO2 flux measurement other than instrumental?

I am working on a instrument called LICOR-8100A soil CO2 flux system but their are some problem in their functioning. so I am looking for a alternative method. If someone have solution of this...

02 February 2023 1,636 2 View

How to dye gelMA hydrogels?

I am synthesizing gelatin methacryloyl hydrogels. I dropcasy my prepolymer mix onto a chip with micro wells and photo polymerize the gels. I want to detect the presence of this gel in the...

22 July 2021 6,374 5 View

Any eye-tracking/self-paced reading (SPR) study where processing of case marker has been the main focus of the research?

I am looking for a research paper recommendation where the researchers focused on the processing of case-markers. Such as Yokoyama et al., 2012, where the research focus is all about the...

25 September 2020 6,314 0 View

How to focus the Confocal Raman Microscope?

I am working with biological samples(mammalian cell lines) to acquire their Raman spectra. I have Raman substrates (gold coated with glass) on top of which I have a polyacrylamide hydrogel. My...

28 August 2020 6,815 7 View

Picogreen Assay for DNA quantification: Which one is better to go for, a fresh cell lysate or a stored cell lysate?

I am working on MSCs culture and to normalise the data, I am doing Picogreen DNA quantification. However, the total amount of DNA is downgraded by 30% when I repeated the quantification using...

26 August 2020 5,322 2 View

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Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Standard curve of H2O2?

Hi, I need to prepare a standard curve of H2O2 to evaluate the primary products of oxidation. H2O2 I have is 35%. How do I prepare a stock of H2O2 10 microlitere. If s.o has experience, I would be...

26 July 2024 3,460 2 View

Can primary care be sustainable ? And how ?

I am starting to work on this question, with particular reference to what we might be able to learn from countries outside the UK. Of course this topic is huge, and I am breaking sustainability...

25 July 2024 1,545 3 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

Cresyl violet Nissl Staining ?

Kindly confirm how to distinguish glia cells and neurons using cresyl violet staining. Also, using cresyl violet nissl staining how to identify the apoptotic neurons ? Does this stain stains...

18 July 2024 2,420 0 View

Can we preserve the protoplasts?

Can we preserve the isolated protoplasts for future experiments? Is it possible to preserve them via cryopreservation? If yes how long can we preserve them?

16 July 2024 9,576 1 View

Trouble finding an anti-Digoxigenin antibody to perform Southern Blot technique?

I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...

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