Trouble polymerizing acrylamide-bisacrylamide gels?

I have been working on making Polyacrylamide gels from 30% Acrylamide and 2% bisacryalmide stock solutions. I prepare the polymer solutions in a centrifuge rube, add APS and TEMED to the solution,...

17 March 2020 5,180 3 View

How to section stem cell spheroid ?

Hi everyone ! Any tips and tricks to section 3D culture/ stem cell spheroid of 1K cells density using Histo gel or Agar gel ?

14 March 2020 2,418 3 View

Which dye is more sensitive, phenol red or thymol blue? and why?

I've been using these dyes for bromination activity purpose and it would be of great help as to if anyone can tell me out of phenol red and thymol blue which dye is more sensitive and why?

11 March 2020 7,477 5 View

Exact ratio of sodium benzoate for medicine preparation ?

what should be the ratio of sodium benzoate in preparing a herbal suspension ?

02 March 2020 6,070 3 View

Which hurdle/ factors mainly restrict the free access to research papers ?

Which hurdle/ factors mainly restrict the free access to research papers ?

17 November 2018 7,689 6 View

For the carbon material prepared, an unusal peak at 2theta =14 degree in the XRD pattern is seen. What is the reflection plane corresponding to that?

I am attaching the XRD pattern of two of my carbon materials C1 and C2. The C2 carbon shows XRD pattern with a broad peak around 15-25 degree whereas C1 shows two distinct peaks in the same...

26 August 2018 1,516 3 View

How to separate blood clot from gel in a serum separator tube?

Hello, I collect blood in gel & clot activator tube for serum separation and I want to extract DNA from clot but unable to separate blood clot from gel as the blood clot lies at the bottom of...

18 December 2017 3,841 2 View

How to solve low undetectable levels of basal ERK phosphorylation in cells?

Hi, I am trying to look for basal ERK phosphorylation in N2a cells. Although, I am able to detect very good intensity of bands for total ERK, but the p-ERK bands are hardly visible. Also, when...

14 December 2017 821 4 View

Why are 2D spots coming in short pH range?

Hello Researchers, I am working with yeast whole proteome but after a lot try I only get spots in region between pH 4-6 using pH 3-10, 7cm strip, following BIO-RAD standard protocol. What could be...

05 June 2016 5,294 11 View

Hepatic cell line expressing TAT?

does anyone have first hands-on/practical experience of working woth any hepatic cell line that expresses tyrosine aminotransferase such that activity is measurable. Also, is activity...

29 May 2016 1,134 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Anyone plz suggest relevant topic for research in image processing+Deep learning.?

I have selected brain tumor images ...but now found that already lots of research done n this topic.

03 March 2021 5,774 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View